Description: This is just a short excerpt for the about page.
Cellular Indexing of Transcriptomes and Epitopes by Sequencing ( CITE-seq ) is a multimodal single cell phenotyping method developed in the Technology Innovation lab at the New York Genome Center in collaboration with the Satija lab.
CITE-seq uses DNA-barcoded antibodies to convert detection of proteins into a quantitative, sequenceable readout. Antibody-bound oligos act as synthetic transcripts that are captured during most large-scale oligodT-based scRNA-seq library preparation protocols (e.g. 10x Genomics, Drop-seq, ddSeq).
This allows for immunophenotyping of cells with a potentially limitless number of markers and unbiased transcriptome analysis using existing single-cell sequencing approaches.
Cell Hashing | CITE-seq
Single Cell Biology | CSHL Single Cell Core Facility | Woodbury
Cell Hashing | CITE-seq
Center for Integrated Cellular Analysis
CITE-seq | A collection of resources for performing CITE-seq
ASAP-seq | CITE-seq