doclu.com - DOCLU: Mplex Multiplexing Software

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Mplex is a semi automated software for evaluation of multiplexed bead assays in flow cytometry. It is particularly useful for cytokine evaluation and can be used with a variety of currently available commercial multiplex kits. It can also be used for lab-designed kits using a combination of beads. Examples shows include kits from BiolegendTM (2 size beads, up to 7 bead intensity levels for 13 plex) and MilliplexTM bead assays kits based on standard LuminexTM beads. MPLEX can easily handle both 96 well and 3

This software was developed to solve a simple problem. How to define the changes in plague coverage on teeth over many months of repeated measurement. First the species was identified then type of tooth structure was developed. An algorithm was developed to create a pixel defined area for plaque density and then an automated algorithm developed to copy that process across similar teeth over time. Data were then plotted monthly to determine the percentage change in plaque coverage.

PlateAnalyzer™ is a unique software package that can read virtually any flow cytometry file type up to 384 well plates and can reduce the complex data to graphically reduced analysis in a few seconds. It can produce IC50 curves (using multiple algorithms) and can perform very complex associations between samples to produce a “what if” type of analysis not possible with any other software approach. There are unlimited number of gating possibilities and virtually any type of designed gate can be created. Down