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Fetal Calf Serum (FCS, also known as Fetal Bovine Serum, or FBS) is a common supplement of animal cell culture media. However, moral and scientific concerns demonstrate the urgency to switch to an FCS-free medium. The FCS-free Database (RRID:SCR_018769), part of the 3Rs Database Programme, provides an overview of FCS-free media for cell-culture.